Amy Rose was captured by Dr. Eggman and his new partner Dr. Wily, along with Tails, Knuckles, and Shadow and all of them were turned into Roboticized Masters. Rose Woman was sent with the others to steal a Chaos Emerald from the Mega City Bank, where they were confronted by Proto Man. Rose Woman and Knuckles Man quickly overpowered their attacker, but Rose Woman was then attacked by Robot Masters, she and her teammates escaped through a Warp Ring with Metal Sonic's assistance. The group briefly split up before rendezvousing -minus Tails Man- back at the Wily Egg and presenting the Emerald to the doctors.
Their primary mission accomplished, Rose Woman and her fellow Masters were sent to attack Sonic, Mega Man and the rest of their party when they managed to enter the Skull Egg Zone. After briefly battling the team of heroes, she and most of her comrades were then dispatched to pursue Proto Man. The task was eventually left to her and Knuckles Man alone while the other remaining Masters were dispatched on other assignments.
When confronting Sonic and Mega Man, Rose Woman attempted to eliminate the heroes by activating a self-destruct mechanism which, even if she failed to terminate her opponents, would deny Sonic the chance of saving Amy. Fortunately, Mega Man was able to disable Rose Woman with the Fire Tornado ability he copied from Blaze Woman, then used his modified Mega Buster to de-roboticize her.
Under normal circumstances, Amy wielded a magical Piko Piko Hammer in combat which delivered immensely powerful strikes. As Rose Woman, she had two hammers, one on each arm.
Rose Woman easily held her own against Sonic and Mega Man during their battle until Mega Man used the Fire Tornado weapon he had obtained from Blaze Woman. This weapon disabled her in one blast and then Mega Man restored Amy to normal with his Mega Buster.