During the Worlds Collide event, spy and jewel thief Rouge the Bat entered the Skull Egg Zone and infiltrated the Wily Egg to spy on Dr. Eggman and Dr. Wily. Inside the fortress, she discovered a Prison Egg containing Wily's former colleague turned rival, Dr. Light, and shared with him a schematic of the Wily Egg she had downloaded from the station's computer. During her investigation of the station, Rouge was cornered by Bass, Metal Sonic and the Chaos Devil. She was then roboticized and joined Dr. Wily's army of Robot Masters that engaged Sonic, Mega Man and their allies during the massive battle outside the fortress.
During the battle, Rouge Woman battled against Knuckles the Echidna. While a formidable opponent, she was overpowered by the combined assault of Sonic and Mega Man. While Sonic grabbed Rouge Woman's legs to weigh her down, Mega Man used the Sonic Shot to turn Rouge back to normal.
Rouge Woman possesses the same flight and fighting abilities as her organic self. Her primary weapon is the Black Wave which fires a burst of dark energy.
Rouge Woman was de-roboticized by the Sonic Shot, though Mega Man did not use any of the weapons he had obtained from the other Roboticized Masters against her.